Plasmid virulence gene expression induced by short-chain fatty acids in Salmonella dublin: identification of rpoS-dependent and rpo-S-independent mechanisms.

نویسندگان

  • A El-Gedaily
  • G Paesold
  • C Y Chen
  • D G Guiney
  • M Krause
چکیده

The Salmonella plasmid virulence spvABCD genes are growth phase regulated and require RpoS for maximal expression in stationary phase. We identified a growth phase-independent expression of spv which is mediated by short-chain fatty acids. During this fatty acid-mediated expression of spv, RpoS is required for induction only during exponential phase. In stationary phase, an rpoS-independent mechanism is responsible for expression of spv.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Analysis of rpoS mRNA in Salmonella dublin: identification of multiple transcripts with growth-phase-dependent variation in transcript stability.

In Salmonella dublin, rpoS encodes an alternative sigma factor of the RNA polymerase that activates a variety of stationary-phase-induced genes, including some virulence-associated genes. In this work, we studied the regulation and transcriptional organization of rpoS during growth. We found two transcripts, 2.3 and 1.6 kb in length, that represent the complete rpoS sequence. The 2.3-kb transcr...

متن کامل

Effects of feed restriction and dietary fat type on mRNA expression of liver fatty acid-binding protein (L-FABP) in broilers

Background: Liver fatty acid-binding protein (L-FABP) is the main cytosolic binding site for long chain fatty acids in hepatocytes. FABPs enhance the uptake of fatty acids into the cell by increasing their concentration due to decreasing concentration of unbound fatty acids inside the cell. Objectives: The aim of this study was to evaluate the effects of dietary unsaturated to saturated fatty a...

متن کامل

Identification and Discrimination of Salmonella Enteritidis, S. Pullorum, S. Gallinarum and S. Dublin Using Salmonella Specific Genomic Regions Amplification Assay

Background: DNA amplification method has been developed for identifying and discriminating Salmonella serovars, using specific primers at the genus and serovar levels and to identify the S. Enteritidis, S. Dublin, S. Gallinarum and S. Pullorum. Objectives: This study was conducted for molecular identification and discrimination among some important Salmonella serovars. Methods: Fifty isolates o...

متن کامل

Probiotic Bifidobacterium Lactis Bacteria Inhibit the Invasion Phenotype of Shigella Dysenteriae Induced By Invasion Plasmid Antigen C

Background and Aims: Shigellosis is an acute gastroenteritis and Invasion plasmid antigen C (IpaC) is the first effector protein for Shigella invasion of intestinal cells. Among lactic acid bacteria, Bifidobacterium lactis (B. lactis) has received increasing attention for protection of a potential host against gastrointestinal infections. The aim of this study was to investigate the inhibitory ...

متن کامل

The Salmonella typhimurium katF (rpoS) gene: cloning, nucleotide sequence, and regulation of spvR and spvABCD virulence plasmid genes.

The spv region of Salmonella virulence plasmids is essential for the development of a systemic infection in mice. Transcriptional activation of the spvABCD operon occurs during stationary growth phase and is mediated by the regulatory gene product SpvR. We have previously shown that expression of a spvRAB'-cat fusion in Escherichia coli was dependent on the katF (rpoS) locus which encodes an al...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of bacteriology

دوره 179 4  شماره 

صفحات  -

تاریخ انتشار 1997